RELA Antibody

• Catalog Number: orb1253833
• Category: Antibodies
• Description: RELA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: NFkB-p65 (Phospho-Thr254) antibody was raised against a peptide sequence around phosphorylation site of threonine 254 (F-R-T (p) -P-P) derived from Human NFκB-p65.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: RELA
• UniProt ID: Q04206
• NCBI: NP_001138610.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: p65, NFKB3, CTNNB, OK/SW-cl.35, PRO2286, RELA, TF6
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from 3T3 cells untreated or treated with TNF-α using NFλB-p65 (Phospho-Thr254).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFλB-p65 (Phospho-Thr254). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa using NFλB-p65 (Phospho-Thr254).