RecombinantShh(C25II),Mouse

• Catalog Number: orb1494773
• Category: Proteins
• Description: Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant mouse Sonic Hedgehog (C25II) (rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rmShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: 19.7 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: IIG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT LGASGRYEGK ITRNSERFKE LTPNYNPDII FKDEENTGAD RLMTQRCKDK LNALAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA VDITTSDRDR SKYGMLARLA VEAGFDWVYY ESKAHIHCSV KAENSVAAKS GG
• Source: Escherichia coli.
• Biological Activity: ED50 500 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant mouse Sonic Hedgehog (C25II) (rmShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: SHH, HHG-1
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.