RecombinantSCF,Rat

• Catalog Number: orb1494609
• Category: Proteins
• Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant rat Stem Cell Factor (rrSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 164 amino acids. A fully biologically active molecule, rrSCF has a molecular mass of 18.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% by SDS-PAGE analysis.
• MW: 18.3 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: QEICRN PVTDNVKDIT KLVANLPNDY MITLNYVAGM DVLPSHCWLR DMVTHLSVSL TTLLDKFSNI SEGLSNYSII DKLGKIVDDL VACMEENAPK NVKESLKKPE TRNFTPEEFF SIFNRSIDAF KDFMVASDTS DCVLSSTLGP EKDSRVSVTK PFMLPPVA
• Source: P. pastoris
• Biological Activity: ED50 4× 10ˆ4 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant rat Stem Cell Factor (rrSCF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.