RecombinantSCF,Human(P.pastoris-expressed)

• Catalog Number: orb1494611
• Category: Proteins
• Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.Recombinant human Stem Cell Factor (rhSCF) produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ~20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 10 mM acetic acid.
• Purity: > 95% by SDS-PAGE analysis.
• MW: ~20 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVA
• Source: P. pastoris
• Biological Activity: The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×10ˆ5 IU/mg.
• Endotoxins: < 1.0 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Stem Cell Factor (rhSCF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhSCF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 10 mM acetic acid.
• Alternative names: C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.