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RecombinantPDGF-BB,Rat
Catalog Number: orb1494777
| Catalog Number | orb1494777 |
|---|---|
| Category | Proteins |
| Description | Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 20 mM acetic acid. |
| Purity | > 95% by SDS-PAGE analysis. |
| MW | 24.6 kDa, observed by non-reducing SDS-PAGE. |
| Protein Sequence | MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPVFKKAT VTLEDHLACK CETVVTPRPV T |
| Source | Escherichia coli. |
| Biological Activity | ED50 5 × 10ˆ5 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 20 mM acetic acid. |
| Alternative names | PDGF Read more... |
| Note | For research use only |
| Application notes | Reconstituted in 20 mM acetic acid at 100 μg/mL. |
| Expiration Date | 6 months from date of receipt. |
