RecombinantPDGF-BB,Human(P.pastoris-expressed)

• Catalog Number: orb1494612
• Category: Proteins
• Description: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle, connective tissue, bone and cartilage cells, and some blood cells. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer.Recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) produced in Pichia pastoris is a glycosylated polypeptide. rhPDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids) and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 10mM acetic acid.
• Purity: > 97% by SDS-PAGE and HPLC analyses.
• MW: 24.3kDa, observed by non-reducing SDS-PAGE.
• Protein Sequence: SL GSLTIAEPAM IAECKTRTEV FEISRRLIDR TNANFLVWPP CVEVQRCSGC CNNRNVQCRP TQVQLRPVQV RKIEIVRKKP IFKKATVTLE DHLACKCETV AAARPVT
• Source: P. pastoris
• Biological Activity: ED50 3.3 x 10ˆ5 units/mg.
• Endotoxins: < 1.0 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhPDGF-BB should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 10mM acetic acid.
• Alternative names: PDGF
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.