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RecombinantOSM,Rat
Catalog Number: orb1494781
| Catalog Number | orb1494781 |
|---|---|
| Category | Proteins |
| Description | Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells. Recombinant Rat Oncostatin M (rrOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 215 amino acids. A fully biologically active molecule, rrOSM has a molecular mass of 24.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| MW | 24.5 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MKRGCSSSS PKLLSQLKSQ ANITGNTASL LEPYILHQNL NTLTLRAACT EHPVAFPSED MLRQLSKPDF LSTVHATLGR VWHQLGAFRQ QFPKIQDFPE LERARQNIQG IRNNVYCMAR LLHPPLEIPE PTQADSGTSR PTTTAPGIFQ IKIDSCRFLW GYHRFMGSVG RVFEEWGDGS RRSRRHSPLW AWLKGDHRIR PSRSSQSAML RSLVPR |
| Source | Escherichia coli. |
| Biological Activity | ED50 1× 10ˆ5 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant Rat Oncostatin M (rrOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrOSM remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | Oncostatin M Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
