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RecombinantNT-4,Human
Catalog Number: orb1494786
| Catalog Number | orb1494786 |
|---|---|
| Category | Proteins |
| Description | Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 50mM acetic acid. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| MW | 28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers. |
| Protein Sequence | MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA |
| Source | Escherichia coli. |
| Biological Activity | ED50 2.0× 10ˆ2 units/mg. |
| Endotoxins | < 0.3 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 50mM acetic acid. |
| Alternative names | Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5) Read more... |
| Note | For research use only |
| Application notes | Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
