RecombinantMIF,Human

• Catalog Number: orb1494795
• Category: Proteins
• Description: Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine, existing as a homotrimer in vivo. MIF was originally identified as a T cell derived factor responsible for the inhibition of macrophage migration. However, recently MIF has received much more attention because of its possible roles in angiogenesis and cancer development. MIF is over-expressed in various cancers, including pancreatic, breast, colon, brain, prostate, skin, and lung. The intratumoral expression of MIF is strongly correlated with angiogenic growth factor expression, such as the expression of Interleukin 8 (IL-8) and Vascular Endothelial Growth Factor (VEGF), and with risk of recurrence after resection. Recombinant human Macrophage Migration Inhibitory Factor (rhMIF) produced in E.coli is a single non-glycosylated polypeptide chain containing 115 amino acids. rhMIF has a molecular mass of 12.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• MW: 12.5 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MPMFIVNTN VPRASVPDGF LSELTQQLAQ ATGKPPQYIA VHVVPDQLMA FGGSSEPCAL CSLHSIGKIG GAQNRSYSKL LCGLLAERLR ISPDRVYINY YDMNAANVGW NNSTFA
• Source: Escherichia coli.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Macrophage Migration Inhibitory Factor (rhMIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Macrophage Migration, Inhibitory Factor, GLIF, MMI
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.