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RecombinantLIX/CXCL5(74aa),Mouse
Catalog Number: orb1494689
| Catalog Number | orb1494689 |
|---|---|
| Category | Proteins |
| Description | Mouse LIX (C-X-C motif chemokine 5) is a small cytokine belonging to the CXC chemokine family that is cleaved into the following 2 chains [GCP-2(1-78) and GCP-2(9-78)]. Mouse LIX plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which could be used to understand more about pain in other inflammatory conditions. It is most closely related to two highly homologous human neutrophil chemoattractants GCP-2 and ENA-78. The first 78 amino acid residues within the predicted mature mouse LIX shares approximately 61% and 55% amino acid identity with human GCP-2 and ENA-78. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.Recombinant mouse LIX/CXCL5 (74aa) produced in CHO cells is a single polypeptide chain containing 74 amino acids. rmLIX/CXCL5(74aa) has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 98% as analyzed by SDS-PAGE. |
| MW | 8 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | VIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEA PVIKKIIQKILGSDKKKA |
| Source | CHO |
| Biological Activity | The EC50 value of mouse LIX/CXCL5 (74aa) on Caˆ2+ mobilization assay in CHO-K1/Gα15/mCXCR2 cells (human Gα15 and mouse CXCR2 stably expressed in CHO-K1 cells) is less than 200 ng/mL. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant mouse LIX/CXCL5 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution,mouse LIX/CXCL5 should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | C-X-C motif chemokine 5, Small-inducible cytokine Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
