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RecombinantIL-18,Rat
Catalog Number: orb1494831
| Catalog Number | orb1494831 |
|---|---|
| Category | Proteins |
| Description | Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0. |
| Purity | > 95% by SDS-PAGE analyses. |
| MW | 18.4 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MHFGRLHCT TAVIRSINDQ VLFVDKRNPP VFEDMPDIDR TANESQTRLI IYMYKDSEVR GLAVTLSVKD GRMSTLSCKN KIISFEEMNP PENIDDIKSD LIFFQKRVPG HNKMEFESSL YEGHFLACQK EDDAFKLVLK RKDENGDKSV MFTLTNLHQS |
| Source | Escherichia coli. |
| Biological Activity | ED50 500 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0. |
| Alternative names | Interleukin-18, IL18 Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
