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RecombinantIGF-I,Mouse
Catalog Number: orb1494838
| Catalog Number | orb1494838 |
|---|---|
| Category | Proteins |
| Description | Insulin-like Growth Factor I (IGF-I) is a single chain 7 kDa growth-promoting polypeptide originally identified as somatomedin-c. It belongs to the IGF family of peptides, which also includes IGF-II and insulin. The gene expression of IGF-I is mainly regulated by Growth Hormone, and IGF-I executes its functions via signaling through transmembrane tyrosine receptors (IGF Receptors). Most circulating IFG-I is associated with the IGF Binding Protein 3 (IGFBP-3), and the IGFBPs may inhibit the actions of IGFs by competing against the IGF Receptors. IGF-I is active in post-natal and adult animals, and is crucial for somatic growth, as IGF-I null mice show marked retardation in utero. IGF-I is involved in carcinogenesis, and related to prostate cancer as well.Recombinant mouse Insulin-like Growth Factor I (rmIGF-I) produced in E. coli is a single non-glycosylated polypeptide chain containing 71 amino acids. A fully biologically active molecule, rmIGF-I has a molecular mass of 7.8kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 95% by SDS-PAGE analysis. |
| MW | 7.8 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MGPETLCGA ELVDALQFVC GPRGFYFNKP TGYGSSIRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPTK AA |
| Source | Escherichia coli. |
| Biological Activity | ED50 1×10ˆ5 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant mouse Insulin-like Growth Factor I (rmIGF-I) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIGF-I remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/mL. |
| Expiration Date | 6 months from date of receipt. |
