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RecombinantIFN-γ,Mouse
Catalog Number: orb1494842
| Catalog Number | orb1494842 |
|---|---|
| Category | Proteins |
| Description | Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.Recombinant mouse IFN gamma (rmIFN-γ) produced in E.coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 95% as analyzed by reducing SDS-PAGE. |
| MW | 15 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC |
| Source | Escherichia coli. |
| Biological Activity | ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells. |
| Endotoxins | < 1 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | Immune Interferon, type II interferon, T cell inte Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
