RecombinantGRO-β/CINC-3/CXCL2,Rat

• Catalog Number: orb1494851
• Category: Proteins
• Description: Chemokine (C-X-C motif) ligand 2(CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 shares 90% amino acid sequence with CXCL1/GROα. The GRO proteins are chemotactic for neutrophils and basophils and can activate them through their CXCR1 or CXCR2 receptors.Recombinant Rat GRO-β/CINC-3/CXCL2 produced in E.coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rrGRO-β has a molecular mass of 7.6kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE.
• MW: 7.6 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: SE LRCQCLTTLP RVDFKNIQSL TVTPPGPHCA QTEVIATLKD GHEVCLNPEA PLVQRIVQKI LNKGKAN
• Source: Escherichia coli.
• Biological Activity: The EC50 value of Rat GRO-β/CINC-3/CXCL2 on Caˆ2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and rat CXCR2 stably expressed in CHO-K1 cells) is less than 10 ng/ml.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Rat GRO-β/CINC-3°CXCL2 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rat GRO-β/CINC-3°CXCL2 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Growth Regulated Protein/Melanoma Growth Stimulato
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.