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RecombinantGM-CSF(Sargramostim),Human(P.pastoris-expressed)
Catalog Number: orb1494614
| Catalog Number | orb1494614 |
|---|---|
| Category | Proteins |
| Description | Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 10 mM PB, pH 7.0. |
| Purity | > 95 % as analyzed by SDS-PAGE. |
| MW | 26-32kDa, observed by SDS-PAGE. |
| Protein Sequence | MAPARS PSPSTQPWEH VNAIQEALRL LNLSRDTAAE MNETVEVISE MFDLQEPTCL QTRLELYKQG LRGSLTKLKG PLTMMASHYK QHCPPTPETS CATQIITFES FKENLKDFLL VIPFDCWEPV QE |
| Source | P. pastoris |
| Biological Activity | ED50 5x 10ˆ6 units/mg. |
| Endotoxins | < 1.0EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 10 mM PB, pH 7.0. |
| Alternative names | Granulocyte Macrophage Colony Stimulating Factor, Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
