RecombinantGM-CSF,Human

• Catalog Number: orb1494856
• Category: Proteins
• Description: Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities [4].Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells [5].Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E.coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
• Purity: > 95 % as analyzed by SDS-PAGE.
• MW: 14 kDa, observed by SDS-PAGE.
• Protein Sequence: MAPARSPSP STQPWEHVNA IQEARRLLNL SRDTAAEMNE TVEVISEMFD LQEPTCLQTR LELYKQGLRG SLTKLKGPLT MMASHYKQHC PPTPETSCAT QIITFESFKE NLKDFLLVIP FDCWEPVQE
• Source: Escherichia coli.
• Biological Activity: ED50 2.0 x 10ˆ6units/mg
• Endotoxins: < 1 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
• Alternative names: Granulocyte Macrophage Colony Stimulating Factor,
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.