RecombinantG-CSF,Human

• Catalog Number: orb1494860
• Category: Proteins
• Description: Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.
• Form/Appearance: Lyophilized after extensive dialysis against 25mM Tris, pH 8.0.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: 18.8kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MTPLGPASS LPQSFLLKCL EQVRKIQGDG AALQEKLCAT YKLCHPEELV LLGHSLGIPW APLSSCPSQA LQLAGCLSQL HSGLFLYQGL LQALEGISPE LGPTLDTLQL DVADFATTIW QQMEELGMAP ALQPTQGAMP AFASAFQRRA GGVLVASHLQ SFLEVSYRVL RHLAQP
• Source: Escherichia coli.
• Biological Activity: ED50 1.0× 10ˆ7 IU/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 25mM Tris, pH 8.0.
• Alternative names: Granulocyte Colony-Stimulating Factor, CSF-3, CSF3
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.