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RecombinantFGF-basic,Bovine
Catalog Number: orb1494865
| Catalog Number | orb1494865 |
|---|---|
| Category | Proteins |
| Description | Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant bovine Fibroblast Growth Factor-basic (rbFGF-basic) produced in E.coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rbFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 95% by SDS-PAGE analysis. |
| MW | 16.4 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | PAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYSS WYVALKRTGQ YKLGPKTGPG QKAILFLPMS AKS |
| Source | Escherichia coli. |
| Biological Activity | ED50 1 × 10ˆ6 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant bovine Fibroblast Growth Factor-basic (rbFGF-basic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rbFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | Fibroblast Growth Factor-basic, FGF-2, FGF2, HBGF- Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/mL. |
| Expiration Date | 6 months from date of receipt. |
