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RecombinantFGF-9,Human
Catalog Number: orb1494872
| Catalog Number | orb1494872 |
|---|---|
| Category | Proteins |
| Description | Fibroblast Growth Factor-9 (FGF-9) is a heparin binding growth factor that belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development.Recombinant Human Fibroblast Growth Factor-9(rhFGF-9) produced in E.coli is a single non-glycosylated polypeptide chain containing 208 amino acids. A fully biologically active molecule, rhFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against PBS. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| MW | 23.4 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MAPLGEVGN YFGVQDAVPF GNVPVLPVDS PVLLSDHLGQ SEAGGLPRGP AVTDLDHLKG ILRRRQLYCR TGFHLEIFPN GTIQGTRKDH SRFGILEFIS IAVGLVSIRG VDSGLYLGMN EKGELYGSEK LTQECVFREQ FEENWYNTYS SNLYKHVDTG RRYYVALNKD GTPREGTRTK RHQKFTHFLP RPVDPDKVPE LYKDILSQS |
| Source | Escherichia coli. |
| Biological Activity | ED50 5.0× 10ˆ5 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant Human Fibroblast Growth Factor-9(rhFGF-9) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-9 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
| Alternative names | FGF9; Fibroblast Growth Factor-9, GAF (Glia-activa Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/ml. |
| Expiration Date | 6 months from date of receipt. |
