RecombinantFGF-8e,Human

• Catalog Number: orb1494874
• Category: Proteins
• Description: Fibroblast Growth Factor 8e (FGF-8e) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation. Recombinant Human FGF-8e produced in E.coli is a single non-glycosylated polypeptide chain containing 212 amino acids. A fully biologically active molecule, rhFGF-8e has a molecular mass of 24.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE
• MW: 24.3 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MQEGPGRGPA LGRELASLFR AGREPQGVSQ QHVREQSLVT DQLSRRLIRT YQLYSRTSGK HVQVLANKRI NAMAEDGDPF AKLIVETDTF GSRVRVRGAE TGLYICMNKK GKLIAKSNGK GKDCVFTEIV LENNYTALQN AKYEGWYMAF TRKGRPRKGS KTRQHQREVH FMKRLPRGHH TTEQSLRFEF LNYPPFTRSL RGSQRTWAPE PR
• Source: Escherichia coli.
• Biological Activity: ED50 < 2.5µg/ml in the presence of 1 µg/mL heparin, measured in a cell proliferation assay using 3T3.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Human FGF 8e remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human FGF 8e should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Fibroblast Growth Factor-8, FGF8e, AIGF, HBGF-8
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.