RecombinantFGF-8c,Mouse

• Catalog Number: orb1494875
• Category: Proteins
• Description: Fibroblast Growth Factor 8c (FGF-8c) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. In different species, e.g. human and mouse, FGF-8 has 8 different isoforms, from FGF-8a to FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, thus conduct different signaling cascade pathways. FGF-8 has very widespread expression pattern during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression; on the other hand, FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.Recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) produced in E.coli is a single non-glycosylated polypeptide chain containing 247 amino acids. A fully biologically active molecule, rmFGF-8c has a molecular mass of 28.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: 28.2 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MQVRSAAQK RGPGAGNPAD TLGQGHEDRP FGQRSRAGKN FTNPAPNYPE EGSKEQRDSV LPKVTQRHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR
• Source: Escherichia coli.
• Biological Activity: ED50 6.7× 10ˆ3 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-8c should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Fibroblast growth factor 8, FGF-8, Androgen-induce
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.