RecombinantFGF-4,Human

• Catalog Number: orb1494879
• Category: Proteins
• Description: Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E.coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.
• Form/Appearance: Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH 7.5.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: 19.4kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MAPTAPNGT LEAELERRWE SLVALSLARL PVAAQPKEAA VQSGAGDYLL GIKRLRRLYC NVGIGFHLQA LPDGRIGGAH ADTRDSLLEL SPVERGVVSI FGVASRFFVA MSSKGKLYGS PFFTDECTFK EILLPNNYNA YESYKYPGMF IALSKNGKTK KGNRVSPTMK VTHFLPRL
• Source: Escherichia coli.
• Biological Activity: ED502.0× 10ˆ6 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH 7.5.
• Alternative names: FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF.
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.