RecombinantEndostatin,Human

• Catalog Number: orb1494618
• Category: Proteins
• Description: Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues.
• Form/Appearance: The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.
• Purity: Greater than 97% by SDS-PAGE
• MW: ~20.2 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: AH SHRDFQPVLH LVALNSPLSG GMRGIRGADF QCFQQARAVG LAGTFRAFLS SRLQDLYSIV RRADRAAVPI VNLKDELLFP SWEALFSGSE GPLKPGARIF SFDGKDVLRH PTWPQKSVWH GSDPNGRRLT ESYCETWRTE APSATGQASS LLGGRLLGQS AASCHHAYIV LCIENSFMTA SK
• Source: P. pastoris
• Biological Activity: Determined by Endothelial Cell Tube Formation. The cells treated with 10μg/ml Endostatin showed significant anti-angiogenesis effect.
• Endotoxins: Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin.
• Storage: The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws.
• Buffer/Preservatives: The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.
• Alternative names: Endostatin
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.