RecombinantENA78/CXCL5(9-78a.a.),Human

• Catalog Number: orb1494890
• Category: Proteins
• Description: Epithelial cell­derived neutrophil­activating peptide (ENA­78) also known as C-X-C motif chemokine 5(CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties Full length CXCL5 (78 a.a.) is cleaved at the N­terminal end by cathepsin G and chymotrypsin to ENA-74 (74 a.a.) and ENA-70 (70a.a.), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor.Recombinant human ENA-78/CXCL5 (9-78a.a.) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhENA-78/CXCL5 (9-78a.a.) has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE.
• MW: 7.7 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: REL RCVCLQTTQG VHPKMISNLQ VFAIGPQCSK VEVVASLKNG KEICLDPEAP FLKKVIQKIL DGGNKEN
• Source: Escherichia coli.
• Biological Activity: The EC50 value of human ENA78/CXCL5 (9-78 a.a.) on Caˆ2+ mobilization assay in CHO-K1/G15/hCXCR2 cells (human G15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 50 ng/ml.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human ENA78°CXCL5 (9-78a.a.) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human ENA78°CXCL5(9-78) should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Epithelial Neutrophil Activating Peptide-78, CXCL5
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.