RecombinantEGFR,His,Human

SKU: orb1494602

Description

Epidermal Growth Factor Receptor (EGFR) belongs to a family of tyrosine kinase receptors including Human EGF Receptors (HER) 2, 3, and 4 which all play important roles in cell growth and differentiation. Their primary ligands are EGF, Heparin-Binding EGF and Transforming Growth Factor α. Upon ligand binding, EGFR undergoes asymmetric dimerization, composed of an “activator” and a “receiver”. EGFR and its family members are disregulated in numerous cancers. In particular, EGFR is overexpressed in many epithelial solid tumors. Evidence suggests EGFR is an excellent target for pharmacologic intervention in Non Small Cell Lung Cancer (NSCLC) due to its high level of expression and prominent role in tumor growth and metastasis. Recombinant human Epidermal Growth Factor Receptor (rhEGFR) with C-terminal 6xHis-tag produced in Sf9 insect cells is a single glycosylated polypeptide chain containing 627 amino acids. rhEGFR has a molecular mass of 80kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: SF9 insect cells
• Biological Activity: Bioassay data are not available.
• Molecular Weight: 80kDa, observed by reducing SDS-PAGE.
• Protein Sequence: LEEK KVCQGTSNKL TQLGTFEDHF LSLQRMFNNC EVVLGNLEIT YVQRNYDLSF LKTIQEVAGY VLIALNTVER IPLENLQIIR GNMYYENSYA LAVLSNYDAN KTGLKELPMR NLQEILHGAV RFSNNPALCN VESIQWRDIV SSDFLSNMSM DFQNHLGSCQ KCDPSCPNGS CWGAGEENCQ KLTKIICAQQ CSGRCRGKSP SDCCHNQCAA GCTGPRESDC LVCRKFRDEA TCKDTCPPLM LYNPTTYQMD VNPEGKYSFG ATCVKKCPRN YVVTDHGSCV RACGADSYEM EEDGVRKCKK CEGPCRKVCN GIGIGEFKDS LSINATNIKH FKNCTSISGD LHILPVAFRG DSFTHTPPLD PQELDILKTV KEITGFLLIQ AWPENRTDLH AFENLEIIRG RTKQHGQFSL AVVSLNITSL GLRSLKEISD GDVIISGNKN LCYANTINWK KLFGTSGQKT KIISNRGENS CKATGQVCHA LCSPEGCWGP EPRDCVSCRN VSRGRECVDK CNLLEGEPRE FVENSECIQC HPECLPQAMN ITCTGRGPDN CIQCAHYIDG PHCVKTCPAG VMGENNTLVW KYADAGHVCH LCHPNCTYGC TGPGLEGCPT NGPKIPSHHH HHH
• Purification: > 95% by SDS-PAGE and HPLC analyses.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human Epidermal Growth Factor Receptor (rhEGFR) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhEGFR remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

ErbB1, HER1