You have no items in your shopping cart.
Cart summary
RecombinantBMP-3B,Human
Catalog Number: orb1494902
| Catalog Number | orb1494902 |
|---|---|
| Category | Proteins |
| Description | Bone Morphogenetic Protein-3B (BMP-3B) , also known as Growth/Differentiation Factor 10 (GDF-10), is a cytokine belonging to the Transforming Growth Factor β (TGF-β) superfamily. BMP-3B contains the cystine knot motif shared by other TGF-β family members. BMP-3B was originally identified by PCR based on the BMP-3 sequence, and shares 83% identity with BMP-3. BMP-3B and BMP-2 act as mutual antagonists, as they compete for the availability of signaling protein Smad4. In vivo, BMP-3B is highly expressed in brain, lungs, and bone tissues. The functions of BMP-3B include acting as a dorsaling factor in head development, inhibition of adipogenesis in adipocytes, and induction of bone formation. BMP-3B is down-regulated in lung cancer patient samples, indicating its potential antitumor activity. Recombinant human Bone Morphogenetic Protein-3B (rhBMP-3B) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 111 amino acids each. rhBMP-3B has a molecular mass of 25.1 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 4mM HCl. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| MW | 25.1 kDa, observed by non-reducing SDS-PAGE. |
| Protein Sequence | MQWDEPRVC SRRYLKVDFA DIGWNEWIIS PKSFDAYYCA GACEFPMPKI VRPSNHATIQ SIVRAVGIIP GIPEPCCVPD KMNSLGVLFL DENRNVVLKV YPNMSVDTCA CR |
| Source | Escherichia coli. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Bone Morphogenetic Protein-3B (rhBMP-3B) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhBMP-3B remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 4mM HCl. |
| Alternative names | Osteogenin, BMP-3A, BMP3B, BMP3A Read more... |
| Note | For research use only |
| Application notes | Reconstituted in 4mM HCl at 100 μg/mL. |
| Expiration Date | 6 months from date of receipt. |
