RecombinantBetacellulin,Mouse

• Catalog Number: orb1494904
• Category: Proteins
• Description: Betacellulin is a pleiotropic cytokine that belongs to the Epidermal Growth Factor (EGF) family. Like other members of the EGF family, Betacellulin possesses a conserved sequence of 35-40 amino acids which contain 3 disulfide bonds formed by 6 cysteines. Betacellulin is unique in the EGF family since it can bind and activate a broad spectrum of ErbB receptors. Functionally, Betacellulin plays a role in the development of the pancreas by activating signaling pathways beneficial for the function, survival and regeneration of pancreatic β-cells. Additionally, Betacellulin has potential angiogenic activities and is important for the growth, development and repair of certain tissues.Recombinant mouse Betacellulin (rmBetacellulin) produced in E.coli is a single non-glycosylated polypeptide chain containing 81 amino acids. A fully biologically active molecule, rmBetacellulin has a molecular mass of 9.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 50mM Tris, 300mM NaCl, pH 9.0.
• Purity: > 95% by SDS-PAGE analysis.
• MW: 9.2 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MDGNTTRTP ETNGSLCGAP GENCTGTTPR QKVKTHFSRC PKQYKHYCIH GRCRFVVDEQ TPSCICEKGY FGARCERVDL FY
• Source: Escherichia coli.
• Biological Activity: ED50 2× 10ˆ6 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant mouse Betacellulin (rmBetacellulin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmBetacellulin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 50mM Tris, 300mM NaCl, pH 9.0.
• Alternative names: BTC
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.