Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

• Catalog Number: orb607796
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Vimentin (Mesenchymal Cell Marker)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: VIM/1937R
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Equine, Feline, Gallus, Human, Porcine
• Isotype: IgG
• Immunogen: Recombinant full-length human Vimentin protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 57-60kDa
• Target: VIM
• Entrez: 7431
• UniProt ID: P08670
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: VIM
• Note: For research use only
• Application notes: Positive Control: U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
• Expiration Date: 12 months from date of receipt.