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Catalog Number | orb1494603 |
---|---|
Category | Proteins |
Description | Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.Recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rhVEGF-A165 has a molecular mass of 38.2kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 38.2kDa, observed by non-reducing SDS-PAGE |
Protein Sequence | APMAEG GGQNHHEVVK FMDVYQRSYC HPIETLVDIF QEYPDEIEYI FKPSCVPLMR CGGCCNDEGL ECVPTEESNI TMQIMRIKPH QGQHIGEMSF LQHNKCECRP KKDRARQENP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR |
Source | P. pastoris |
Biological Activity | ED50 of 1-5ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2x 10ˆ5-1x 10ˆ6 units/mg. |
Endotoxins | < 0.5 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhVEGF-A165 should be stable up to 4 week at 4°C or up to 6 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. |
Alternative names | Vascular Endothelial Growth Factor, VPF, Folliculo Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
CLIA, EIA, LF | |
> 90% as determined by SDS-PAGE | |
20.8 kDa | |
HEK293 |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2113, Recombinant Human VEGF165 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala27-Arg191) of Human VEGF165 fused with no tag . |
> 95% by Tris-Bis PAGE | |
KMP2112, Biotinylated Recombinant Human VEGF165 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala27-Arg191) of Human VEGF165 fused with a His Tag and Avi tag at the C-terminal. |
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