Recombinant TNF-α, Human

• Catalog Number: orb1494766
• Category: Proteins
• Description: Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.Recombinant Human Tumor Necrosis Factor-alpha (TNF-α) produced in E.coli is a single non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rhTNF-α has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 17.3 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: VRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
• Source: Escherichia coli.
• Biological Activity: ED50 3.3 x 10ˆ7 units/mg.
• Endotoxins: Less than 0.2 EU/μg determined by LAL test.
• Storage: Lyophilized recombinant Human Tumor Necrosis Factor-alpha (TNF-α), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: TNF-alpha, TNF-α; Tumornecrosis factor ligand supe
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.