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Catalog Number | orb1494770 |
---|---|
Category | Proteins |
Description | Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 28.4 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | 0 |
Source | Escherichia coli. |
Biological Activity | 6 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C. |
Storage | Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles. |
Buffer/Preservatives | Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH 7.5. |
Alternative names | rTEV, TEV, P1 protease Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
Greater than 90.0% as determined by RP-HPLC and analysis by SDS-PAGE |
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