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Catalog Number | orb1516638 |
---|---|
Category | Proteins |
Description | Recombinant SARS-Cov-2 Spike RBD protein, mFc (HEK293) |
Tag | mFc |
Concentration | > 0.5 mg/ml |
Purity | > 90% as determined by SDS-PAGE |
MW | 60 kDa |
Target | Spike glycoprotein |
Protein Sequence | 319-541/1273 |
Expression System | HEK293 cell |
Endotoxins | Not analyzed |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike RBD (WT), mFc Tag (Cat: orb1516638), the EC50 is 34 ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
65 kDa |
98.00% | |
51.78 kDa (predicted); 56.20 kDa (reducing condition, due to glycosylation) |