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Catalog Number | orb1516626 |
---|---|
Category | Proteins |
Description | Recombinant SARS-Cov-2 (Omicron, B.1.1.529) Spike trimer protein, C-His (HEK293) |
Tag | C-His |
Concentration | > 0.5 mg/ml |
Purity | > 90% as determined by SDS-PAGE |
MW | 150-180 kDa |
Target | Spike glycoprotein |
Protein Sequence | 16-1213/1273 |
Expression System | HEK293 cell |
Endotoxins | Not analyzed |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Alternative names | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/mL (100 µl/Well) can bind SARS-CoV-2 Spike trimer Protein (His Tag) (Omicron/B.1.1.529) (Cat: orb1516626), the EC50 is 1.5 µg/mL.
Measured by its binding ability in a indirect ELISA. Immobilized SARS-CoV-2 Spike trimer Protein (His Tag) (Omicron/B.1.1.529) (Cat: orb1516626) at 2 µg/mL (100 µl/Well) can bind Anti-SARS-CoV-2 Spike Monoclonal antibody.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
98.00% | |
136.70 kDa (predicted). Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |