Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

• Catalog Number: orb2316010
• Category: Antibodies
• Description: Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rS100A4/1481
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 10-12kDa
• Target: S100A4
• Entrez: 6275
• UniProt ID: P26447
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: S100 calcium-binding protein A4; CAPL; Fibroblast
• Note: For research use only
• Application notes: Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).
• Expiration Date: 12 months from date of receipt.