Recombinant PD-L1 antibody [AC37]

• Catalog Number: orb1090509
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PD-L1
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: AC37
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:100-1:400
• Form/Appearance: Purified recombination monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide, and 0.05% rAlbumin. This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 33275
• Target: CD274
• UniProt ID: Q9NZQ7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Programmed cell death 1 ligand 1 antibody, an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PDL-1 Antibody at 1:1000 dilution. Lane 1: HEK 293 transfected with PDL1 whole cell lysate. Lane 2: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG (H&L) HRP. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)

Immunohistochemical analysis of paraffin-embedded human esophageal squamous carcinoma tissue. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)

Immunohistochemical analysis of paraffin-embedded NCI-H226 (left) and HEK 293 transfected with PD-L1 (right). Cell was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L)