Recombinant NT-4, Human

• Catalog Number: orb1494786
• Category: Proteins
• Description: Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.
• Protein Sequence: MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA
• Source: Escherichia coli.
• Biological Activity: ED50 2.0× 10ˆ2 units/mg.
• Endotoxins: < 0.3 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 50mM acetic acid.
• Alternative names: Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5)
• Note: For research use only
• Application notes: Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml.
• Expiration Date: 6 months from date of receipt.