Recombinant Murine GRO/KC/ CXCL1 (rMuKC/CXCL1)

• Catalog Number: orb1494925
• Category: Proteins
• Description: KC coded by CXCL1 gene at chromosome 5 is approximately 63% identity to that of mouse MIP2. KC is also approximately 60% identical to the human GROs. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease.
• Form/Appearance: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 97% by SDS-PAGE and HPLC analyses.
• MW: Approximately 7.8kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues.
• Protein Sequence: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK
• Source: Escherichia coli.
• Biological Activity: Measured by its ability to chemoattract human CXCR2 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 3-15 ng/mL.
• Endotoxins: Less than 1EU/μg of rMuKC/CXCL1 as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.