Recombinant Murine Eotaxin/CCL11 (rMuEotaxin/CCL11)

• Catalog Number: orb1494927
• Category: Proteins
• Description: Eotaxin also called CCL11 is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and along Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung, and in allergic reactions.
• Form/Appearance: Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• MW: Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
• Protein Sequence: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
• Source: Escherichia coli.
• Biological Activity: Measured by its ability to chemoattract mouse CCR3 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 1-5 ng/mL.
• Endotoxins: Less than 1EU/μg of rMuEotaxin/CCL11 as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.