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Catalog Number | orb1673143 |
---|---|
Category | Proteins |
Description | Recombinant Mouse LIF |
Concentration | < 1.0 EU/μg of recombinant protein as determined by the LAL method. |
Form/Appearance | Recombinant Leukemia Inhibitory Factor was lyophilized from a 0.2 μm filtered PBS pH 7.5. |
Purity | > 95% as determined by SDS-PAGE |
MW | 20 kDa |
Solubility (25°C) | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Source | CHO cells |
Biological Origin | Mouse |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Alternative names | LIF, Differentiation-stimulating factor, D factor, Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA | |
> 95% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
> 95% by SDS-PAGE. | |
KMP1638, Recombinant Mouse LIF/Leukemia inhibitory factor Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser24-Phe203) of mouse LIF/Leukemia inhibitory factor (Accession #P09056). |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Lif(Ser24-Phe203) was fused without Tag and expressed in E. coli. |
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