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Catalog Number | orb1975970 |
---|---|
Category | Proteins |
Description | Recombinant mouse IgG1 Fc protein, (HEK293) |
Clonality | Recombinant |
Tag | Tag Free |
Concentration | > 1mg/ml |
Purity | > 90% as determined by SDS-PAGE |
MW | 25.7 kDa |
Target | Ig gamma-1 chain C region secreted form |
Protein Sequence | 98-324/324 |
Expression System | HEK293 cell |
Endotoxins | Not tested |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | Ig gamma-1 chain C region secreted form, Ighg1 Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
Unconjugated | |
95% | |
24.3 kDa | |
Mouse IgG Fc, Tag Free (orb257565) is expressed from human 293 cells (HEK293). It contains AA Val 111 - Lys 324 (Accession # AAK53870.1). |
98.00% | |
25.8 kDa (predicted); 32 kDa (reducing condition, due to glycosylation) |
> 95% as determined by SDS-PAGE | |
32 kDa |
98.00% | |
75.4 kDa (predicted) |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 34-36 kDa based on Tris-Bis PAGE result. kDa |