Recombinant LR3-IGF-I (Receptor Grade), Human

• Catalog Number: orb1494802
• Category: Proteins
• Description: IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 9.1±0.9 KDa, observed by reducing SDS-PAGE.
• Protein Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
• Source: Escherichia coli.
• Biological Activity: ED50 ＜10 ng/ml, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of > 1.0× 10ˆ5 units/mg.
• Endotoxins: ＜1 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80°C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized after dissolved in 48%acetonitrile and 0.1% TFA
• Alternative names: Human LR3 Insulin-like Growth Factor-I (LR3-IGF-I)
• Note: For research use only
• Application notes: Reconstituted in 10mM HCl at 1mg/ml. Use a 0.22 μm membrane for filter Sterilization.
• Expiration Date: 6 months from date of receipt.