Recombinant LIF, Mouse, AF

• Catalog Number: orb1178930
• Category: Proteins
• Description: Recombinant LIF, Mouse, AF
• Tested applications: ELISA
• Reactivity: Mouse
• Tag: His-tag at the N-terminus
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SDS-PAGE. Ni-NTA chromatography.
• Entrez: 16878
• Protein Sequence: SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF with polyhistidine tag at the N-terminus.
• Source: Escherichia coli
• Biological Activity: Measure by its ability to induce IL-6 secretion in M1 cells. The ED50 for this effect is < 0.5 ng/mL. The specific activity of recombinant mouse LIF is > 2 x 106 IU/mg.
• Endotoxins: < 0.1 EU per 1 μg of the protein by the LAL method.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Buffer/Preservatives: The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
• Alternative names: Differentiation-stimulating factor, D factor, Mela
• Note: For research use only
• Expiration Date: 6 months from date of receipt.