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Catalog Number | orb1494804 |
---|---|
Category | Proteins |
Description | Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E.coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 19.7 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | SPL PITPVNATCA IRHPCHNNLM NQIRSQLAQL NGSANALFIL YYTAQGEPFP NNLDKLCGPN VTDFPPFHAN GTEKAKLVEL YRIVVYLGTS LGNITRDQKI LNPSALSLHS KLNATADILR GLLSNVLCRL CSKYHVGHVD VTYGPDTSGK DVFQKKKLGC QLLGKYKQII AVLAQAF |
Source | Escherichia coli. |
Biological Activity | ED50 5 × 10ˆ6 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | D factor, MLPLI Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/mL. |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
48.6 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses | |
20.3 kDa |
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