Recombinant IL-5, Rat

• Catalog Number: orb1494815
• Category: Proteins
• Description: Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.Recombinant rat Interleukin-5 (rrIL-5) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 113 amino acids each. A fully biologically active molecule, rrIL-5 has a molecular mass of 26.0 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 26.0 kDa, observed by non-reducing SDS-PAGE.
• Protein Sequence: MEIPMSTVV KETLIQLSTH RALLTSNETM RLPVPTHKNH QLCIGEIFQG LDILKNQTVR GGTVEILFQN LSLIKKYIDG QKEKCGEERR KTRHFLDYLQ EFLGVMSTEW AMEV
• Source: Escherichia coli.
• Biological Activity: ED50 2.5× 10ˆ6 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant rat Interleukin-5 (rrIL-5) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 20mM Tris, pH 8.5.
• Alternative names: Interleukin-5, IL5
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.