Recombinant IL-3β, Rat

• Catalog Number: orb1494818
• Category: Proteins
• Description: Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant rat Interleukin-3 beta (rrIL-3 beta) produced in E.coli is a single non-glycosylated polypeptide chain containing of 144 amino acids. A fully biologically active molecule, rrIL-3 beta has a molecular mass of 16.3 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 16.3 kDa, observed by non-reducing SDS-PAGE.
• Protein Sequence: MISDRGSDA HHLLRTLDCR TIALEILVKL PYPQVSGLNN SDDKANLRNS TLRRVNLDEF LKSQEEFDSQ DTTDIKSKLQ KLKCCIPAAA SDSVLPGVYN KDLDDFKKKL RFYVIHLKDL QPVSVSRPPQ PTSSSDNFRP MTVEC
• Source: Escherichia coli.
• Biological Activity: ED50 2 × 10ˆ5 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant rat Interleukin-3 beta (rrIL-3 beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-3 beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Interleukin-3 beta; IL3β
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.