Recombinant IL-18, Rat

• Catalog Number: orb1494831
• Category: Proteins
• Description: Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 18.4 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MHFGRLHCT TAVIRSINDQ VLFVDKRNPP VFEDMPDIDR TANESQTRLI IYMYKDSEVR GLAVTLSVKD GRMSTLSCKN KIISFEEMNP PENIDDIKSD LIFFQKRVPG HNKMEFESSL YEGHFLACQK EDDAFKLVLK RKDENGDKSV MFTLTNLHQS
• Source: Escherichia coli.
• Biological Activity: ED50 500 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0.
• Alternative names: Interleukin-18, IL18
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.