Recombinant IL-1β, Rat

• Catalog Number: orb1494826
• Category: Proteins
• Description: Interleukin-1 Beta (IL-1 Beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant rat Interleukin-1 Beta (rat IL-1 Beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 152 amino acids. A fully biologically active molecule, recombinant rat IL-1 Beta protein has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 17.3 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: VPI RQLHCRLRDE QQKCLVLSDP CELKALHLNG QNISQQVVFS MSFVQGETSN DKIPVALGLK GLNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKTKVEFE SAQFPNWYIS TSQAEHRPVF LGNSNGRDIV DFTMEPVSS
• Source: Escherichia coli.
• Biological Activity: ED50 1.0× 10ˆ8 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant rat Interleukin-1 Beta (IL-1 Beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant rat IL-1 Beta should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Interleukin-1β; IL-1β; Catabolin, Lymphocyte-activ
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.