Recombinant IGF-I (N-Met), Human

• Catalog Number: orb1494840
• Category: Proteins
• Description: Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which are differentially expressed by various tissues. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but only IGF-II can bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults. Recombinant Human IGF-I(N-Met) produced in E.coli is a polypeptide chain containing 71 amino acids. A fully biologically active molecule, rhIGF-I (N-Met) has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 7.8 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MGPETLCGA ELVDALQFVC GDRGFYFNKP TGYGSSSRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPAK SA
• Source: Escherichia coli.
• Biological Activity: ED50 2.0× 10ˆ5 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Human IGF-I(N-Met) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human IGF-I should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.