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Catalog Number | orb1494839 |
---|---|
Category | Proteins |
Description | Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which is differentially expressed by various tissues. Mature human IGF-I respectively shares 94% and 96% aa sequence identity with mouse and rat IGF-I. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but IGF-II can alone bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults. Recombinant human Insulin-like growth factor I (rhIGF-I) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhIGF-I has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 7.7 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
Source | Escherichia coli. |
Biological Activity | ED50 2.0× 10ˆ5 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Insulin-like growth factor I (rhIGF-I) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIGF-I should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 7743 Da, a single non-glycosylated polypeptide chain containing 70 amino acids. 15N stable isotope labeled. | |
Escherichia coli |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 67 amino acids. | |
Escherichia coli |
> 97% as determined by SDS-PAGE and HPLC. | |
7.4 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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