Recombinant IFN-γ, Mouse

• Catalog Number: orb1494842
• Category: Proteins
• Description: Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers.Recombinant mouse IFN gamma (rmIFN-γ) produced in E.coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 15 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC
• Source: Escherichia coli.
• Biological Activity: ED50<0.15ng/ml, measured by cytotoxicity assay using WEHI-279 cells.
• Endotoxins: < 1 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Immune Interferon, type II interferon, T cell inte
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.